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KMID : 0895820110210040049
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Journal of Oriental Rehabilitation Medicine
2011 Volume.21 No. 4 p.49 ~ p.65
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Effects of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang ) Treatment on the Monosodium Iodoacetate-induced Early Stage Osteoarthritis in Rats
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Park Dong-Su
Jeong Su-Hyeon
Kim Soon-Joong
Seo Il-Bok
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Abstract
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Objectives: This study was performed to investigate the effects of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) on the monosodium iodoacetate(MIA) induced early stage osteoarthritis in rats.
Methods: Osteoarthritis was induced by injection of MIA(0.25 mg) into knee joints of rats. Osteoarthritic rats were divided into control(n=8) and treated(n=8) group respectively. Control group was taken distilled water and treated group was taken extracts of Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) by orally for 20 days. Body weight was measured at 0, 5, 10, 15, 20 days after MIA injection. At the end of experiment, gross and histopathological examination on the articular cartilages of the knee joints were performed. Proteoglycan(PG) content of articular cartilages were analysed by safranine O staining method. The content of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) in synovial fluids were analysed by enzyme-linked immunosorbent assay(ELISA) method. And also cyclooxygenase-2(COX-2), matrix metalloproteinase 3(MMP-3), inducible nitric oxide synthase(iNOS), calpain immunohistochemical examination on the knee joints were performed.
Results: PG content in articular cartilages of the treated group was significantly increased compared with control group. Histopathological osteoarthritic score of the treated group was significantly decreased compared with control group. TNF-α content in synovial fluids, expression of iNOS and calpain in synovial membrane of the treated group were significantly decreased compared with control group. But body weight, IL-1β content in synovial fluids, expression of iNOS and MMP-3 of the treated group were not significantly changed compared with control group.
Conclusions: On the basis of these results, we conclude that Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang) has anti-arthritic effects on the MIA induced early stage osteoarthritis in rats.
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KEYWORD
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Gagamsosokmyeong-tang(Jiajianxiaoxuming-tang), Proteoglycan, TNF-α, iNOS, Calpain, Anti-arthralgic effect
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